Open Access, Peer-reviewed

ISSN 2005-7571 (Online)
Volume 27, Number 1 (1/2020)
Original Article <page. 27-35 >
DOI : 10.22857/kjbp.2020.27.1.004

Ketamine-Induced Behavioral Effects Across Different Sub-Anesthetic Dose Ranges in Adolescent and Adult Mice

Hyung Jun Choi, DVM1;Soo Jung Im, MS1;Hae Ri Park, AS1;Seong Mi Lee, MS1;Chul-Eung Kim, MD2; and Seunghyong Ryu, MD3;

1;Department of Mental Health Research, National Center for Mental Health, Seoul, 2;Mental Health Research Institute, National Center for Mental Health, Seoul, 3;Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea

Objectives : Ketamine has been reported to have antidepressant effects or psychotomimetic effects. The aim of this study was to investigate the behavioral effects of ketamine treatment at various sub-anesthetic doses in adolescent and adult naïve mice.

Methods : In each experiment for adolescent and adult mice, a total of 60 male Institute of Cancer Research mice were randomly divided into 6 groups, which were intraperitoneally treated with physiological saline, 10, 20, 30, 40, and 50 mg/kg ketamine for consecutive 3 days. At 1 day after last injection, the locomotor and depressive-like behaviors were evaluated in mice, using open field test (OFT) and forced swim test (FST), respectively.

Results : In case of adolescent mice, ketamine dose was negatively correlated with total distance traveled in the OFT (Spearman's rho = -0.27, p = 0.039). In case of adult mice, we found significant positive correlation between ketamine dose and duration of immobility in the FST (Spearman's rho = 0.45, p < 0.001). Immobility time in the 50 mg/kg ketamine-treated mice was significantly higher compared to the saline-treated mice (Dunnett's post-hoc test, p = 0.012).

Conclusions : We found that the repeated treatment with ketamine could decrease the locomotor or prolong the duration of immobility in mice as the dose of ketamine increased. Our findings suggest that sub-anesthetic doses of ketamine might induce schizophrenia-like negative symptoms but not antidepressant effects in naïve laboratory animals.

Key words : Ketamine;Mouse;Open field test;Forced swim test.


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